Matrix Assisted Laser Desorption (MALDI), a versatile tool for the characterization of Dendrimers

Matrix-Assisted Laser Desorption/Ionization (MALDI)

Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry

A highly enriched spindle pole preparation was prepared from budding yeast and fractionated by SDS gel electrophoresis. Forty-five of the gel bands that appeared enriched in this fraction were analyzed by high-mass accuracy matrix-assisted laser desorption/ ionization (MALDI) peptide mass mapping combined with sequence database searching. This identified twelve of the known spindle pole compone...

Direct Matrix-Assisted Laser Desorption-Ionisation (MALDI) Mass-Spectrometry Bacteria Profiling for Identifying and Characterizing Pathogens

This study examines the features and limitations of direct Matrix-Assisted Laser Desorption-Ionisation (MALDI) mass-spectrometry profiling of bacterial cells for investigating a microbial population. The optimal laboratory protocol, including crude bacteria lyses by a solution of 50% acetonitrile, 2.5% trifluoroacetic acid, and using α-cyano-4-hydroxy cinnamic acid as the MALDI matrix, has been...

Electrospray sample deposition for matrix-assisted laser desorption/ionization (MALDI) and atmospheric pressure MALDI mass spectrometry with attomole detection limits.

Electrospray sample deposition was explored for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). In this method, nanoliter volumes of matrix/analyte mixture were electrosprayed from a high voltage biased (1-2 kV) fused-silica capillary onto a grounded MALDI plate mounted 100-500 microm from the capillary outlet. Electrospray deposition with these condi...

Matrix-assisted laser desorption ionization time-of-flight mass-spectroscopy (MALDI-TOF MS)

10 # Corresponding Author: Mailing address: Karen C. Carroll, Johns Hopkins Hospital 11 Microbiology Laboratory, Meyer B1-193, 600 N. Wolfe St., Baltimore MD 21287; 410-95512 5077; FAX 410-614-8087; [email protected] 13 Copyright © 2012, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.01405-12 JCM Accepts, published online ahead of print on 1 August 2012